| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209637 |
| Chromosome: | chromosome 8 |
| Location: | 1931062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367300 | ESS101 | (1 of 2) PF17035 - Bromodomain extra-terminal - transcription regulation (BET) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCCTGGGGAATGCATCCCTACAGCAGG |
| Internal bar code: | TGGCCCGTGCGGCCCGAGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.937 |
| LEAP-Seq n confirming: | 1586 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAATTGGTAATGCACG |
| Suggested primer 2: | CGAACAGTCATAGCCCTGGT |