| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209669 |
| Chromosome: | chromosome 6 |
| Location: | 7142739 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297500 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAACTGCATGCGCCAACTCTTGCGTCGC |
| Internal bar code: | GTAGAACCTCTTAAATGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACCACCATGCGACTTT |
| Suggested primer 2: | GCTGCTATCTTTGGCCTTTG |