| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209687 |
| Chromosome: | chromosome 14 |
| Location: | 1113536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615150 | (1 of 26) IPR001202 - WW domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACAACATGTCAACACGCACGGCATGGA |
| Internal bar code: | ACGGATGCGTGCAAGCAAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 81.6216 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGGTGTGACTAATCCAG |
| Suggested primer 2: | CCTTGTAGCAACTGAACGCA |