| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209689 |
| Chromosome: | chromosome 4 |
| Location: | 2408979 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221250 | (1 of 1) PF11378 - Protein of unknown function (DUF3181) (DUF3181) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGTCCGCGTCTTCAAGCGTCTCGTGTTC |
| Internal bar code: | AATCAATCGACGCTTCAACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 94.2593 |
| LEAP-Seq n confirming: | 509 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGGTGAGCTTGAGAGCA |
| Suggested primer 2: | ACACACACACTTCCTCGCTG |