Insertion junction: LMJ.RY0402.209704_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.209704
Chromosome:chromosome 16
Location:2375109
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g659950 PRPS5 Chloroplast Ribosomal Protein S5; (1 of 2) K02988 - small subunit ribosomal protein S5 (RP-S5, MRPS5, rpsE) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATTCTGACACCAGATCTACAACACCGCC
Internal bar code:CGTAGAAGTCAAGTGGAAGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:685
LEAP-Seq percent confirming:99.3816
LEAP-Seq n confirming:2732
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCGCAAGACTGATTGACT
Suggested primer 2:CAGCCGGTCATAAACCTTGT