| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209707 |
| Chromosome: | chromosome 1 |
| Location: | 4303782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029000 | COQ5D,UMM1,COQD2 | (1 of 2) PTHR10108//PTHR10108:SF813 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTGGGCATCCTCAGTGCCTGCACCTG |
| Internal bar code: | CCCGTGTCTTCGCAGTTTTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.128 |
| LEAP-Seq n confirming: | 9663 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCTATGGTAGGCACGG |
| Suggested primer 2: | ACTACATGACCCGCCTTTTG |