| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209722 |
| Chromosome: | chromosome 16 |
| Location: | 213416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694750 | GT90F30,GT90-30 | GT90 family protein 30; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAACATTACGCTAGGTGTGTGTATGTG |
| Internal bar code: | AGTTTAGTGACTTGGTTGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.7375 |
| LEAP-Seq n confirming: | 760 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAACCCCTCTGTCGAAC |
| Suggested primer 2: | GCACAATGTCCCTACACACG |