Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.209723 |
Chromosome: | chromosome_11 |
Location: | 3434533 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g481000 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCACGACACACTCTCCAGCCACCACAGCC |
Internal bar code: | GATGCACGCGCCGGAGTATTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 592 |
LEAP-Seq percent confirming: | 96.1737 |
LEAP-Seq n confirming: | 9149 |
LEAP-Seq n nonconfirming: | 364 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGATGCAGAGCAGCAATGTA |
Suggested primer 2: | GAGGAGGAGAAGGAGGAGGA |