| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209739 |
| Chromosome: | chromosome 12 |
| Location: | 2740683 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503300 | (1 of 1) 4.1.1.21 - Phosphoribosylaminoimidazole carboxylase / AIR carboxylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAACGTGTGGGCGCCGTACGACGCGCCC |
| Internal bar code: | GCAAGTCTGTCAGACGGTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 98.0574 |
| LEAP-Seq n confirming: | 2322 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTACACGCAGGAGATG |
| Suggested primer 2: | CAGCCTTTTTCCTCCTACCC |