| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209751 |
| Chromosome: | chromosome 17 |
| Location: | 3917477 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728400 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCAGCCGCCAGCTGCGCCGCCAGCGCC |
| Internal bar code: | AGGGCCCGTACAAATCAGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 98.8839 |
| LEAP-Seq n confirming: | 3721 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCTGCTCACGCACACAC |
| Suggested primer 2: | TTACTGCATCTGCGGTCTTG |