| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209758 |
| Chromosome: | chromosome 3 |
| Location: | 6648501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197050 | HTV2 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCTCAACAATTGACTATGTTGTCGCTG |
| Internal bar code: | AGCGGCTCAAGAACAACTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.8442 |
| LEAP-Seq n confirming: | 1922 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGCAGCTCCGTTGTCTT |
| Suggested primer 2: | TCATAGCCCAGCTGTGTCAG |