Insertion junction: LMJ.RY0402.209784_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.209784
Chromosome:chromosome_10
Location:1637065
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g429750 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CCATCGCAAACTCCACCCGGCCCGCTCCAA
Internal bar code:CACTCTCCCCCCCGATCGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:505
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGGAACATTGGAGGAGT
Suggested primer 2:CGGCAGTCATCTTCTTCACA