| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209787 |
| Chromosome: | chromosome 10 |
| Location: | 1798729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430950 | RAD54B,SRH18 | (1 of 1) K10875 - DNA repair and recombination protein RAD54 and RAD54-like protein (RAD54L, RAD54); SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTCGTAGCTGATGATGAGCACCCGGT |
| Internal bar code: | CGGTTCGCGGGTCGTATCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 99.7995 |
| LEAP-Seq n confirming: | 2986 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCATAACTGCATAAGGGC |
| Suggested primer 2: | TGGCTTCCCTATCCAGTACG |