| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209796 |
| Chromosome: | chromosome 9 |
| Location: | 2924028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388050 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATTGTACTGAGCGATATACAGTATTT |
| Internal bar code: | GAAGAGTTACTGTTGGCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.4 |
| LEAP-Seq n confirming: | 4142 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGAATGCGTGACATCCG |
| Suggested primer 2: | CCTGTACAGCTCCACAAGCA |