| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209804 |
| Chromosome: | chromosome 3 |
| Location: | 6282974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192950 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCTGCCTAGCGTCAAGCTGCTGGGGCA |
| Internal bar code: | GTCTGAGTTGATGTTGGGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.7494 |
| LEAP-Seq n confirming: | 2786 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCGTGTTCGTTCAGGC |
| Suggested primer 2: | CTGTGCCTGTTCCTCTCCTC |