| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209809 |
| Chromosome: | chromosome_12 |
| Location: | 5787319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g533351 | CLPB5 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCGCTCCGTTCTCCACATGAACAGCTAAC |
| Internal bar code: | ACGCACAGAAAAGCACGGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 99.1022 |
| LEAP-Seq n confirming: | 39298 |
| LEAP-Seq n nonconfirming: | 356 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCGGGTTGTTCTTTGT |
| Suggested primer 2: | GAGGGTCAGTGTGTGTGTGG |