| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209824 |
| Chromosome: | chromosome 6 |
| Location: | 1999579 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264150 | MAW11 | Membrane-associated hydroxyproline-rich glycoprotein 11; (1 of 1) 2.1.1.53 - Putrescine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGTGAGTGCTGTCGCCTTTGTCAGTC |
| Internal bar code: | CCAGGGCAAGCAACCGCTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 98.1919 |
| LEAP-Seq n confirming: | 706 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACTATGCTCGCCTGCAA |
| Suggested primer 2: | TGAGAATGTTGCTGAGTGGC |