| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209828 |
| Chromosome: | chromosome_9 |
| Location: | 2181000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g393150 | FOX1 | Multicopper ferroxidase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAAACAATGCATACCGTCGCTCGCGCTCC |
| Internal bar code: | TATCTCGACTGAACGACGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 99.1 |
| LEAP-Seq n confirming: | 2973 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGTTGGACAGGTATGG |
| Suggested primer 2: | CTGATCTGGATGGAGTCGGT |