Insertion junction: LMJ.RY0402.209883_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.209883
Chromosome:chromosome 8
Location:2546060
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g372300 (1 of 1) IPR000104//IPR000620 - Antifreeze protein, type I // EamA domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGTGATCGGCGGTGTTGAGGAGCGAGGC
Internal bar code:TGCTCCGGTGGTATTCAACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:447
LEAP-Seq percent confirming:99.5703
LEAP-Seq n confirming:8574
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCTCCTCCTTCGTCTC
Suggested primer 2:GGATGGGCTGTCAGTTGTCT