| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209886 |
| Chromosome: | chromosome 10 |
| Location: | 32307 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCTCATCGTCACTACCAGGCACTGCTC |
| Internal bar code: | TTGGCGATGCGTCCGGTGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 12.2329 |
| LEAP-Seq n confirming: | 521 |
| LEAP-Seq n nonconfirming: | 3738 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGAAGTTGTGATTCCGT |
| Suggested primer 2: | ATGATGTCACCTGGTCGTGA |