| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209906 |
| Chromosome: | chromosome_17 |
| Location: | 4448159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g732150 | FAP130 | Flagellar Associated Protein | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTATGATGCAGTGTTGGGCGCGAGAAGCG |
| Internal bar code: | TGGGGGCCAAGCGATATTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.1681 |
| LEAP-Seq n confirming: | 2265 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGCTGTAGAGTGGCAG |
| Suggested primer 2: | ACTTGTATGTGGGCAGCCTC |