| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209920 |
| Chromosome: | chromosome 10 |
| Location: | 3364125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444000 | (1 of 2) IPR007743//IPR027417//IPR030385 - Immunity-related GTPases-like // P-loop containing nucleoside triphosphate hydrolase // IRG-type guanine nucleotide-binding (G) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGGAATATCTTGACCTTGTTGGCGTCA |
| Internal bar code: | GAACTTCCGGGGCTAAACTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 98.6456 |
| LEAP-Seq n confirming: | 437 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAATTCACTAACGGGGTG |
| Suggested primer 2: | GCAAGTGACAACCTCAAGCA |