| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.209925 |
| Chromosome: | chromosome_1 |
| Location: | 53395 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g000250 | SNR1 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTTGTAGCTCTGATCGCTGTAGCCCAGAT |
| Internal bar code: | GGGCGGTTGGTGGCGTCGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 50.5494 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGGTAGACGTAGGCAAA |
| Suggested primer 2: | CTATTCGTTCTTTCCGCTGC |