Insertion junction: LMJ.RY0402.209925_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.209925
Chromosome:chromosome 1
Location:53400
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g000250 SNR1 (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX; Predicted SNARE-associated Golgi protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAACAGCCGGCACCCACCGTACAGGGC
Internal bar code:AAAAGAACAGCGGAGAGGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:96.3225
LEAP-Seq n confirming:2724
LEAP-Seq n nonconfirming:104
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGGTAGACGTAGGCAAA
Suggested primer 2:CTATTCGTTCTTTCCGCTGC