| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209968 |
| Chromosome: | chromosome 12 |
| Location: | 6423369 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537750 | (1 of 1) PTHR22957:SF34 - TBC1 DOMAIN FAMILY MEMBER 25 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGAGTGCAGACGCCACACGCGAGCTT |
| Internal bar code: | CGGACTTTGGTTGCGGGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 98.3103 |
| LEAP-Seq n confirming: | 640 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCAACAAACTCCTCCAT |
| Suggested primer 2: | GACCTCTTCTGACGCCAGTC |