Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.209982 |
Chromosome: | chromosome_10 |
Location: | 2269343 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434400 | PF6 | Flagellar central pair-associated protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACGCCACGACAGTGAGTGCCGCTGGAACG |
Internal bar code: | CCGATGAGGAAAGAGCGCTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 513 |
LEAP-Seq percent confirming: | 99.7802 |
LEAP-Seq n confirming: | 5448 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAGCGTTGCTCTTGTGTA |
Suggested primer 2: | TTGAATCAGGTCATTCACGG |