| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209982 |
| Chromosome: | chromosome 10 |
| Location: | 2269343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g434400 | PF6 | (1 of 1) K19090 - CRISPR-associated protein Cas5t (cas5t); Flagellar central pair-associated protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCCACGACAGTGAGTGCCGCTGGAACG |
| Internal bar code: | CCGATGAGGAAAGAGCGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 5448 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGCGTTGCTCTTGTGTA |
| Suggested primer 2: | TTGAATCAGGTCATTCACGG |