| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209990 |
| Chromosome: | chromosome 4 |
| Location: | 1190524 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCGCACCGCCCCCGCCCCCATCACT |
| Internal bar code: | AGGATCGCTGTCCCCCTTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 33.5664 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACTCAAGCGTTCATGC |
| Suggested primer 2: | TTACAGACTGCACAGCCCAG |