| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209993 |
| Chromosome: | chromosome 12 |
| Location: | 5887503 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAACTCCGGGACCCGGGGACCCGCACC |
| Internal bar code: | CGAATGTGGGTTGGGGGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 2472 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAACCCCATCCAGCTA |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |