| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210026 |
| Chromosome: | chromosome 2 |
| Location: | 7361841 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145000 | RBF1 | ribosome-binding factor A; (1 of 1) K02834 - ribosome-binding factor A (rbfA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCACGCGCTTGCCCGCCACTGCCCGG |
| Internal bar code: | CTCCCTTTTTTTACGAGGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 99.6036 |
| LEAP-Seq n confirming: | 1005 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGATGTCGCTGGCTTA |
| Suggested primer 2: | CTCCTCCTCCCTTAGTCGCT |