| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210029 |
| Chromosome: | chromosome 12 |
| Location: | 1189740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489800 | (1 of 4) PF00887 - Acyl CoA binding protein (ACBP) | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAACTAATGCGAACGTAGGCTGCAAGG |
| Internal bar code: | GTGTTCGCATTTAGCTTTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.0559 |
| LEAP-Seq n confirming: | 2518 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCCATATCTTTCTCGC |
| Suggested primer 2: | CTCAACACGACACCATCCAC |