Insertion junction: LMJ.RY0402.210043_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210043
Chromosome:chromosome 11
Location:1156718
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467686 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTAGCAGTGGCGCGTGCGGTTTCAACG
Internal bar code:GGGGTCTGAGCCGTCGCGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:140
LEAP-Seq percent confirming:98.9407
LEAP-Seq n confirming:467
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACACGAGGGTTGAAAAACG
Suggested primer 2:CATGGTTTGCAGGACAGATG