| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210065 |
| Chromosome: | chromosome 12 |
| Location: | 6471678 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538250 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCGTGCCACCGCTCCACGAAACACCA |
| Internal bar code: | CCTCGTTTTTCAAGTGGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.5228 |
| LEAP-Seq n confirming: | 2711 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTACGCACAAAAGGATG |
| Suggested primer 2: | ACTGGTGCTGTCAGTGTTGC |