| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210070 |
| Chromosome: | chromosome 3 |
| Location: | 5001308 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180400 | (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGGCATGAGTGGAGGCCATTGTCGGT |
| Internal bar code: | CAGAGGCGGGGGGTTACGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 396 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGCTACTCACGTTCGAC |
| Suggested primer 2: | TCTTGGCGTTGATGTAGCTG |