| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210085 |
| Chromosome: | chromosome 7 |
| Location: | 567586 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTGCAGATGGACCGCTTGGCCTCGTT |
| Internal bar code: | ACGTGGTTCGGAGCTTACCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 99.0916 |
| LEAP-Seq n confirming: | 3818 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACGACGGAGAGCAGGAC |
| Suggested primer 2: | CGTAAAAGCGCATTAGGAGC |