| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210088 |
| Chromosome: | chromosome 6 |
| Location: | 3995665 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278198 | (1 of 1) 1.14.11.33//4.2.99.18 - DNA oxidative demethylase / Alkylated DNA repair protein // DNA-(apurinic or apyrimidinic site) lyase / Phage-T4 UV endonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGAAGTCGTAGCCAGGTATAAAGGTA |
| Internal bar code: | TGTCAGCTATCTTGGGCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 99.6441 |
| LEAP-Seq n confirming: | 560 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGAGTGAGGATGAGGT |
| Suggested primer 2: | GCCAGGCTATAAGAAGTGCG |