| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210093 |
| Chromosome: | chromosome 11 |
| Location: | 2050318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468600 | Hypothetical protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACGTAGCGTGTTTCCGTTTACCTACT |
| Internal bar code: | GAACCAGCGCAGATGTATGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 99.6689 |
| LEAP-Seq n confirming: | 301 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTAACGCACACACACGCA |
| Suggested primer 2: | GAGTGTGGGAGTGGGAGTGT |