| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210121 |
| Chromosome: | chromosome_1 |
| Location: | 4896199 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g033950 | EXN2 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGTGGGTGGCTGCCTGCTTGGGAGTGCA |
| Internal bar code: | GACCAACGCGGATCATGGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 50.8287 |
| LEAP-Seq n confirming: | 276 |
| LEAP-Seq n nonconfirming: | 267 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGAAAGGACTGTGTGA |
| Suggested primer 2: | AAAACCGCCATCTGACTTTG |