| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210141 |
| Chromosome: | chromosome 16 |
| Location: | 5312434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682350 | (1 of 1) PTHR11685:SF154 - E3 UBIQUITIN-PROTEIN LIGASE ARI5-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACCGGCTATCCAGCAACCTCTACGGCG |
| Internal bar code: | TGCCTTTTCAAAGCGGTGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.4398 |
| LEAP-Seq n confirming: | 1775 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATGACTACGGCTTTGCT |
| Suggested primer 2: | ACACTGTCCGCCTCATTACC |