| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210178 |
| Chromosome: | chromosome_10 |
| Location: | 1834587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g431150 | ROC22,FAP256 | Flagellar Associated Protein, involved in circadian rhythms | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACCAGCACGCCGTACTGCACCCCCCAA |
| Internal bar code: | TATTGGGAGAAGAATTGAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.7705 |
| LEAP-Seq n confirming: | 3478 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACAGGGTAGAGCGGGTT |
| Suggested primer 2: | TTGATACTCATGCCAGCGAG |