| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210196 |
| Chromosome: | chromosome 11 |
| Location: | 2635389 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476450 | (1 of 2) PTHR22807//PTHR22807:SF34 - NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING // METHYLTRANSFERASE NSUN6-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGTATGTGGGTGCTGGGCGCCTGCGCC |
| Internal bar code: | CCAACGTGCGAAGGCAGCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 99.6543 |
| LEAP-Seq n confirming: | 28248 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTGGAACCCGTGGTACT |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |