| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210199 |
| Chromosome: | chromosome 17 |
| Location: | 2293623 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713900 | LST8 | TOR kinase binding protein; (1 of 1) K08266 - G protein beta subunit-like (GBL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGGACTTCGCCAGCGTGTTCAGACGAT |
| Internal bar code: | TAGCGGACGGTTTTTGAAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.4302 |
| LEAP-Seq n confirming: | 4886 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGTCCGTCGAAGTTTG |
| Suggested primer 2: | GTGCCTCATATCACCCGACT |