Insertion junction: LMJ.RY0402.210223_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210223
Chromosome:chromosome 12
Location:7947577
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGAGATCGCGCTGTACGACCACAAGGTA
Internal bar code:CGTACCGCCGACGCGGGTCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:318
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGAGAAGGAGAGGGAGA
Suggested primer 2:AAACAGTAGCGCCCTCTTGA