| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210243 |
| Chromosome: | chromosome 10 |
| Location: | 6492442 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466450 | (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTGAAGGGCGCAGGGCGCTTGGAAG |
| Internal bar code: | GGACGGCGTGGTCTTGTACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.5483 |
| LEAP-Seq n confirming: | 1102 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCAGCAAGAAGCAGAAG |
| Suggested primer 2: | AGCACGAAGTCGGAGTCTGT |