| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210250 |
| Chromosome: | chromosome 13 |
| Location: | 662203 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566200 | CGL112 | possible transcription factor; (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCTCTACAGGGTCAGCAGCCATGGACG |
| Internal bar code: | TCGTCATGCAAACCGGCGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.6595 |
| LEAP-Seq n confirming: | 10537 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAGCTCGCTCTCAGTAT |
| Suggested primer 2: | AGTTCGGTGTTATGGGCAAG |