| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210267 |
| Chromosome: | chromosome 10 |
| Location: | 1912946 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGGCGCACTGCGACCTCAAGCCAGCC |
| Internal bar code: | ACCGGTTTCGGATAGGATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.8158 |
| LEAP-Seq n confirming: | 542 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCGGAAACTAGTGAAG |
| Suggested primer 2: | AGGCACTTACTCGACGGAGA |