| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210272 |
| Chromosome: | chromosome 16 |
| Location: | 6095226 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675900 | OAD1 | Arginine/ornithine decarboxylase; (1 of 2) 4.1.1.20 - Diaminopimelate decarboxylase / Meso-2,6-diaminoheptanedioate carboxy-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGATGGAGGGCGGCGGAACAGCTTATT |
| Internal bar code: | GCCATAGGACATTCTGCATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1119 |
| LEAP-Seq percent confirming: | 99.6787 |
| LEAP-Seq n confirming: | 4653 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGAGTACACCAAGCAG |
| Suggested primer 2: | GTATCCCGATAGCGCTGTGT |