| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210280 |
| Chromosome: | chromosome 6 |
| Location: | 8986606 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311650 | IRK1 | (1 of 1) PTHR11767//PTHR11767:SF58 - INWARD RECTIFIER POTASSIUM CHANNEL // FI16807P1; Inwardly-rectifying potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCGTCACGCATGCACAAAGCGGGGCAG |
| Internal bar code: | ATGAATGTATGGTCAACAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 99.5823 |
| LEAP-Seq n confirming: | 6437 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGTACAGGGTGCGTGT |
| Suggested primer 2: | AGAAACCAGACCACCACCTG |