| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210299 |
| Chromosome: | chromosome 16 |
| Location: | 6096614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675900 | OAD1 | Arginine/ornithine decarboxylase; (1 of 2) 4.1.1.20 - Diaminopimelate decarboxylase / Meso-2,6-diaminoheptanedioate carboxy-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTTCTGGGACAAGGCGGAACACCGGTT |
| Internal bar code: | CGTCGAAGCAGGGGGTGACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.7795 |
| LEAP-Seq n confirming: | 905 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACCCATCTGCCTCTAT |
| Suggested primer 2: | GCTACTGCTGCTGCTGCTAC |