| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210306 |
| Chromosome: | chromosome 16 |
| Location: | 5780996 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678400 | (1 of 1) PTHR22847//PTHR22847:SF412 - WD40 REPEAT PROTEIN // TRANSDUCIN/WD40 DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCGCAGCCTCCTCGACAGCAGTAGAC |
| Internal bar code: | GGATGACAAATTCCGTATTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 99.5128 |
| LEAP-Seq n confirming: | 12459 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATGATGCCCTTAATTT |
| Suggested primer 2: | GTTTACTTGGTTCGGGCGTA |