| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210362 |
| Chromosome: | chromosome 12 |
| Location: | 5383630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529800 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGTACATGAGTGATTTCGGCTGGTGG |
| Internal bar code: | CTGCGGACAGATCAGGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 99.6739 |
| LEAP-Seq n confirming: | 917 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCCAGAATACAGCTATG |
| Suggested primer 2: | CTGCTGCAGGTGAGAATGAA |